Interfering with intracellular DNA repair mechanisms can improve CRISPR efficiency – Sohu Technology cibi

DNA repair mechanism of intra cell interference can improve the efficiency of CRISPR? – [Technews] new technology technology Sohu CRISPR-Cas9 system in recent years as the most popular gene editing technology, widely used in academic circles are used in the study, to eliminate specific genes in human cell lines, and to find out the function of the gene, but this there was a place that it removed in want of perfection, the effect of different genes have great difference. Published in August 17th in the journal Science Research Network Nature Communications, a team of researchers from the California University of California at Berkeley, found a new method to enhance CRISPR-Cas9 rejection in various human cells (knockout) gene efficiency to the original 5 times, making the knockout easier, and chances for gene mutation can not operate take this as a medical method. Scientists constantly through various experimental techniques in biological systems in finding new genes, and the proteins they translated out, but the really hard work is to find these genes and proteins play in the formation of organisms or disease in the role. The best way to identify a gene or protein physiological function and role, is to remove it from the organism, see the biological operation what to change, to find out the possible role of unraveling it finally, in other experimental verification method. Since the advent of CRISPR gene editing technology, this technology allows scientists to study, in a shorter period of time to remove specific genes from the cell line. In this process, the researchers must continue to try a variety of gene editing tools to find the best and most suitable for the convenience of the experiment. Because of the stable cell lines used in the study are mostly from cancerous cells, the number of each gene these tumor cell line, usually more than two times more than normal cells, the gene knockout is higher in these stable tumor cell line on the difficulty of the technology improvement will greatly improve the efficiency of such research. In this study, the research team found a way to make the process simple and fast. The key to CRISPR-Cas9 protein and oligonucleotides (oligonucleotides) together into the cell, where the oligonucleotide used is a kind of short DNA sequences, with any fragment and not in the human genome, DNA can repair mechanism of intra cell interference, the CRISPR of gene editing efficiency increase. The lead researcher of the study is as the University of California at Berkeley Innovative Genomics Initiative (IGI) and molecular and cellular biology science assistant professor Jacob Corn, he said, after the strengthening of this technology, excluding specific genes become very easy, as long as the will and human gene summary report相关的主题文章: